赵美萍
教授，博士生导师
研究方向：分析化学
办公室：B333
电话：010-62758153
电子信箱：mpzhao@pku.edu.cn
课题组主页：http://www.chem.pku.edu.cn/mpzhao
学术简历
· 2010 –至今 教授，北京大学化学与分子工程学院
· 2002 –2010 副教授，北京大学化学与分子工程学院
· 1998.7-1998.11 访问学者荷兰能源研究机构环境分析部
· 1997.9–1998.6 访问学者荷兰阿姆斯特丹大学环境与毒理化学系
· 2002 理学博士北京大学化学与分子工程学院
· 1993 理学硕士北京大学环境科学中心
· 1990 理学学士 北京大学 技术物理系
科研领域及兴趣
本课题组致力于探索和构建新颖的分子识别体系，发展实时、在线的荧光分析方法，为生命科学研究、临床检验、环境监测和食品安全监控提供高效的分析测量手段。目前的主要研究方向：
(1) 细胞内核酸修复酶的荧光成像方法；
(2) 低丰度基因突变的超高灵敏检测及其在临床检验中的应用；
(3) 新型功能化分子识别新材料的设计、合成和应用；
(4) 外源基因的识别和定位方法。
代表性论文：
1. JQ Zhai, MH Zhao, XJ Cao, MY Li*, MP Zhao*, Metal-ion-responsive bionanocomposite for selective and reversible enzyme inhibition,Journal of the American Chemical Society,2018, 140, 16925.
2. TB Wu, YF Yang, W Chen, JY Wang, ZY Yang, SL Wang, XJ Xiao, MY Li,MP Zhao*, Noncanonical substrate preference of lambda exonuclease for 5'-nonphosphate-ended dsDNA and a mismatch-induced acceleration effect on the enzymatic reaction,Nucleic Acids Research,2018, 46, 3119.
3. TB Wu, W Chen, ZY Yang, HC Tan, JY Wang, XJ Xiao, MY Li,MP Zhao*, DNA terminal structure-mediated enzymatic reaction for ultra-sensitive discrimination of single nucleotide variations in circulating cell-free DNA,Nucleic Acids Research,2018, 46(4), e24.
4. XJ Xiao, TB Wu, L Xu, W Chen,MP Zhao*, A branch-migration based fluorescent probe for straightforward, sensitive and specific discrimination of DNA mutations,Nucleic Acids Research,2017, 45 (10): e90.
5. JQ Zhai, YB Liu, S Huang, SM Fang,MP Zhao*, A specific DNA-nanoprobe for optically tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells,Nucleic Acids Research,2017, 45(6), e45.
6. XJ Xiao, TB Wu, FD Gu,MP Zhao*, Generation of artificial sequence-specific nucleases via preassembled inert-template,Chemical Science,2016, 7, 2051.
7. SM Fang, L Chen,MP Zhao*, Unimolecular chemically modified DNA fluorescent probe for one-step quantitative measurement of the activity of human apurinic/ apyrimidinic endonuclease 1 in biological samples,Analytical Chemistry,2015, 87, 11952.
8. XJ Xiao, C Zhang, X Su, C Song,MP Zhao*, A universal mismatch-directed signal amplification platform for ultra-selective and sensitive DNA detection under mild isothermal conditions,Chemical Science,2012, 3, 2257.
9. ZY Chen, L Xu, Y Liang,MP Zhao*, pH-Sensitive water-soluble nanospheric imprinted hydrogels prepared as horseradish peroxidase mimetic enzyme,Advanced Materials,2010, 22, 1488.
10. C Song,MP Zhao*, Real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by a singly labeled DNA-hairpin smart probe coupled with l exonuclease cleavage,Analytical Chemistry,2009, 81, 1383.
MeipingZhao
Ph.D. inAnalytical Chemistry, Professor
Email:mpzhao@pku.edu.cn
Office:B333
Office phone:010-6275-8153
Research Experience
2010- Professor, Peking University.
2002- 2010 Associate Professor, Peking University.
1998-Visiting scientist at ECN of the Netherlands.
1997-1998Visiting scientist at MTC of Amsterdam University, the Netherlands.
2002 Ph.D. in Analytical Chemistry, Peking University.
1993 M.S. in Environmental Chemistry, Peking University.
1990 B.S. in Applied Chemistry, Peking University.
Research Areas
The research of our group is mainly focused on exploring the chemical mechanisms of the bio-molecular recognition reactions and trying to simulate the natural process to develop highly selective, sensitive and efficient analytical methods. Current research projects include:
(1)Fluorescence imaging of DNA repair enzymes in living cells；
(2)Ultra-sensitivedetection oflow-abundance DNA mutationsin circulating cell-free DNA；
(3)Mimicking the bio-molecular recognition by molecular imprinting technology；
(4) Identification of the integration sites of exogenous genes in mammalian cells.
Representative Publications
1. JQ Zhai, MH Zhao, XJ Cao, MY Li*, MP Zhao*, Metal-ion-responsive bionanocomposite for selective and reversible enzyme inhibition,Journal of the American Chemical Society,2018, 140, 16925.
2. TB Wu, YF Yang, W Chen, JY Wang, ZY Yang, SL Wang, XJ Xiao, MY Li,MP Zhao*, Noncanonical substrate preference of lambda exonuclease for 5'-nonphosphate-ended dsDNA and a mismatch-induced acceleration effect on the enzymatic reaction,Nucleic Acids Research,2018, 46, 3119.
3. TB Wu, W Chen, ZY Yang, HC Tan, JY Wang, XJ Xiao, MY Li,MP Zhao*, DNA terminal structure-mediated enzymatic reaction for ultra-sensitive discrimination of single nucleotide variations in circulating cell-free DNA,Nucleic Acids Research,2018, 46(4), e24.
4. XJ Xiao, TB Wu, L Xu, W Chen,MP Zhao*, A branch-migration based fluorescent probe for straightforward, sensitive and specific discrimination of DNA mutations,Nucleic Acids Research,2017, 45 (10): e90.
5. JQ Zhai, YB Liu, S Huang, SM Fang,MP Zhao*, A specific DNA-nanoprobe for optically tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells,Nucleic Acids Research,2017, 45(6), e45.
6. XJ Xiao, TB Wu, FD Gu,MP Zhao*, Generation of artificial sequence-specific nucleases via preassembled inert-template,Chemical Science,2016, 7, 2051.
7. SM Fang, L Chen,MP Zhao*, Unimolecular chemically modified DNA fluorescent probe for one-step quantitative measurement of the activity of human apurinic/ apyrimidinic endonuclease 1 in biological samples,Analytical Chemistry,2015, 87, 11952.
8. XJ Xiao, C Zhang, X Su, C Song,MP Zhao*, A universal mismatch-directed signal amplification platform for ultra-selective and sensitive DNA detection under mild isothermal conditions,Chemical Science,2012, 3, 2257.
9. ZY Chen, L Xu, Y Liang,MP Zhao*, pH-Sensitive water-soluble nanospheric imprinted hydrogels prepared as horseradish peroxidase mimetic enzyme,Advanced Materials,2010, 22, 1488.
10. C Song,MP Zhao*, Real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by a singly labeled DNA-hairpin smart probe coupled with l exonuclease cleavage,Analytical Chemistry,2009, 81, 1383.
